Anti-ACSL4/FACL4 Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF ELISA

A04372-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACSL4/FACL4 antigen affinity purified polyclonal antibody (A04372-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACSL4/FACL4 at approximately 75 kDa. The expected band size for ACSL4/FACL4 is at 79, 75 kDa.
all(3)
ICC/IF analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-3).
ACSL4/FACL4 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-3) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(3)
all(3)

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产品简介>

产品名称

Anti-ACSL4/FACL4 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

ACS4; ACSL4; ACSL4/FACL4; FACL4; LACS 4; LACS4; MRX63; MRX68

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, ELISA

基因名称

ACSL4

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human FACL4/ACSL4 recombinant protein (Position: A36-E237).

计算分子量

79, 75 kDa

实际分子量

75 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Long-chain-fatty-acid—CoA ligase 4 is an enzyme that in humans is encoded by the ACSL4 gene. It is mapped to Xq23. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.

Uniprot ID

O60488

文献引用格式

ACSL4/FACL4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A04372-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

acyl-CoA synthetase long chain family member 4

蛋白名全称

Long-chain-fatty-acid--CoA ligase 4

实验方案>

实验步骤

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