Anti-CD11a/Integrin Alpha L/ITGAL Antibody

Rabbit polyclonal antibody

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筛选器： All WB FCM

A04466-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD11a/Integrin Alpha L/ITGAL using anti-CD11a/Integrin Alpha L/ITGAL antibody (A04466-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: JURKAT whole cell lysates,
Lane 2: CEM whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD11a/Integrin Alpha L/ITGAL antigen affinity purified polyclonal antibody (A04466-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD11a/Integrin Alpha L/ITGAL at approximately 180 kDa. The expected band size for CD11a/Integrin Alpha L/ITGAL is at 129 kDa.
all(2)
Flow Cytometry analysis of U937 cells using anti-CD11a antibody (A04466-1).Overlay histogram showing U937 cells stained with anti-CD11a Antibody (A04466-1 (Blue line). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(2)

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产品简介>

产品名称

Anti-CD11a/Integrin Alpha L/ITGAL Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CD11A; Integrin alpha L; ITGAL; LFA 1; LFA 1A; LFA1A

产品类型

Polyclonal

检验物种

human

应用范围

WB, FCM

基因名称

ITGAL

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CD11a recombinant protein (Position: F161-L349). Human CD11a shares 73.7% mino acid (aa) sequence identity with mouse CD11a.

计算分子量

129 kDa

实际分子量

180 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CD11a, also known as ITGAL or LFA-1, is a human gene which functions in the immune system. It is mapped to 16p11.2. CD11a is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab. CD11a encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. It is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1.

Uniprot ID

P20701

文献引用格式

CD11a/Integrin Alpha L/ITGAL Antibody (Boster Biological Technology, Wuhan, China. Catalog#A04466-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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