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Anti-VEGFB Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ELISA

A04494-2

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of VEGFB using anti-VEGFB antibody (A04494-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat heart tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGFB antigen affinity purified polyclonal antibody (A04494-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VEGFB at approximately 19,29 kDa. The expected band size for VEGFB is at 22 kDa.

    all(6)
  • IHC analysis of VEGFB using anti-VEGFB antibody (A04494-2).
    VEGFB was detected in a paraffin-embedded section of mouse heart tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFB Antibody (A04494-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)
  • IHC analysis of VEGFB using anti-VEGFB antibody (A04494-2).
    VEGFB was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFB Antibody (A04494-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)
  • IHC analysis of VEGFB using anti-VEGFB antibody (A04494-2).
    VEGFB was detected in a paraffin-embedded section of rat skeletal muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFB Antibody (A04494-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)
  • IHC analysis of VEGFB using anti-VEGFB antibody (A04494-2).
    VEGFB was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFB Antibody (A04494-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)

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产品简介>

产品名称
Anti-VEGFB Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
vascular endothelial growth factor B; VEGFL; VRF; Vegfb
产品类型
Polyclonal
检验物种
mouse, rat
应用范围
WB, IHC, ELISA
基因名称
Vegfb
宿主
Rabbit
抗体亚型
IgG
免疫原
E. coli-derived rat VEGFB recombinant protein (Position: P22-A207). Rat VEGFB shares 87.1% and 93.5% amino acid (aa) sequence identity with human and mouse VEGFB, respectively.
计算分子量
22 kDa
实际分子量
19,29 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and thecerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.
Uniprot ID
O35485  
文献引用格式
VEGFB Antibody (Boster Biological Technology, Wuhan, China. Catalog#A04494-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
ELISA:1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-VEGFB Antibody

    筛选器: All WB IHC ELISA

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