Anti-EPHB3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A04659

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-EPHB3 antibody (A04659). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse stomach tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EPHB3 antigen affinity purified polyclonal antibody (A04659) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EPHB3 at approximately 110 kDa. The expected band size for EPHB3 is at 110-130 kDa.
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IHC analysis of EPHB3 using anti-EPHB3 antibody (A04659).
EPHB3 was detected in a paraffin-embedded section of human glioma tissue. The tissue section was incubated with rabbit anti-EPHB3 Antibody (A04659) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of EPHB3 using anti-EPHB3 antibody (A04659).
EPHB3 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-EPHB3 Antibody (A04659) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
all(4)

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产品简介>

产品名称

Anti-EPHB3 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

EPHB3; ETK2; HEK2; TYRO6; Ephrin type-B receptor 3; EPH-like tyrosine kinase 2; EPH-like kinase 2; Embryonic kinase 2; EK2; hEK2; Tyrosine-protein kinase TYRO6

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ELISA

基因名称

EPHB3

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human EPHB3 recombinant protein (Position: H275-Q554).

计算分子量

110 kDa

实际分子量

110 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Ephrin Receptor EphB3, is also known as human embryo kinase2(HEK2) or Eph-like tyrosine kinase2(ETK2). HEK2, which is a member of the EPH/ELK family of tyrosine kinases, encodes a 998-amino acid polypeptide having a single putative transmembrane domain, a secretory signal sequence, and 2 fibronectin repeats. The EPHB3 gene is mapped to human chromosome 3q21-qter. HEK2 interacts with 2 ligands of EPH-related kinases(LERKs), namely, LERK2(EFNB1) and LERK5(EFNB2). Coincubation of HEK2- and LERK2-expressing cells induces cell-cell adhesion and aggregation. Additionally, coexpression of HEK2 and LERK2 results in reduced kinase activity of HEK2.

Uniprot ID

P54753

文献引用格式

EPHB3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A04659)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

EPH receptor B3

蛋白名全称

Ephrin type-B receptor 3

实验方案>

实验步骤

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