Anti-Kir6.1/KCNJ8 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM ELISA

A04950-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Kir6.1/KCNJ8 using anti-Kir6.1/KCNJ8 antibody (A04950-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Kir6.1/KCNJ8 antigen affinity purified polyclonal antibody (A04950-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Kir6.1/KCNJ8 at approximately 48 kDa. The expected band size for Kir6.1/KCNJ8 is at 48 kDa.
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IHC analysis of Kir6.1/KCNJ8 using anti-Kir6.1/KCNJ8 antibody (A04950-1).
Kir6.1/KCNJ8 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Kir6.1/KCNJ8 Antibody (A04950-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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Flow Cytometry analysis of U2OS cells using anti-Kir6.1/KCNJ8 antibody (A04950-1).
Overlay histogram showing U2OS cells stained with A04950-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Kir6.1/KCNJ8 Antibody (A04950-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介>

产品名称

Anti-Kir6.1/KCNJ8 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

KCNJ8; Kir6.1; uKATP 1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM, ELISA

基因名称

KCNJ8

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Kir6.1/KCNJ8 recombinant protein (Position: M1-S424). Human KCNJ8 shares 98.3% and 97.6% amino acid (aa) sequence identity with mouse and rat KCNJ8, respectively.

计算分子量

48 kDa

实际分子量

48 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Potassium inwardly-rectifying channel, subfamily J, member 8, also known as KCNJ8, is a human gene encoding the Kir6.1 protein. Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. Defects in this gene may be a cause of J-wave syndromes and sudden infant death syndrome (SIDS).

Uniprot ID

Q15842

文献引用格式

Kir6.1/KCNJ8 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A04950-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

potassium inwardly rectifying channel subfamily J member 8

蛋白名全称

ATP-sensitive inward rectifier potassium channel 8

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实验步骤

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