Anti-Calmegin/CLGN Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A05261-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Calmegin/CLGN using anti-Calmegin/CLGN antibody (A05261-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: H9C2(2-1) whole cell lysates,
Lane 3: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Calmegin/CLGN antigen affinity purified polyclonal antibody (A05261-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calmegin/CLGN at approximately 95 kDa. The expected band size for Calmegin/CLGN is at 70 kDa.
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产品简介>

产品名称

Anti-Calmegin/CLGN Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

calmegin; CLGN

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, ELISA

基因名称

CLGN

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse Calmegin/Clgn recombinant protein (Position: N18-D611).

计算分子量

70 kDa

实际分子量

95 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Calmegin, also known as CLGN, is a protein which in humans is encoded by the CLGN gene. This gene belongs to the calreticulin family, which includes calreticulin, calnexin, and calmegin, and encodes a calcium-binding molecular chaperone specifically expressed in pachytene stage male germ cells. It is required for the proper folding of newly synthesized membrane proteins in the endoplasmic reticulum including those critical for sperm migration from the uterus into the oviduct and sperm adhesion to and penetration of the zona pellucida. This gene plays a key role in spermatogenesis and male infertility. Alternative splice variants exist for this gene.

Uniprot ID

P52194

文献引用格式

Calmegin/CLGN Antibody (Boster Biological Technology, Wuhan, China. Catalog#A05261-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

calmegin

蛋白名全称

Calmegin

实验方案>

实验步骤

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