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Anti-ASGR1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ICC/IF IP FCM

A05376-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of ASGR1 using anti-ASGR1 antibody (A05376-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
    Lane 2: human HepG2 whole cell lysates,
    Lane 3: rat liver tissue lysates,
    Lane 4: mouse liver tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ASGR1 antigen affinity purified polyclonal antibody (A05376-1) at a dilution of 1:2000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ASGR1 at approximately 38 kDa. The expected band size for ASGR1 is at 33 kDa.

    all(7)
  • IHC analysis of ASGR1 using anti-ASGR1 antibody (A05376-1).
    ASGR1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ASGR1 Antibody (A05376-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of ASGR1 using anti-ASGR1 antibody (A05376-1).
    ASGR1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-ASGR1 Antibody (A05376-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of ASGR1 using anti-ASGR1 antibody (A05376-1).
    ASGR1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-ASGR1 Antibody (A05376-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)

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产品名称
Anti-ASGR1 Antibody
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
ASGP R 1; ASGPR; ASGPR 1; Asgpr1; ASGR1; asialoglycoprotein receptor 1; CLEC4H1; Hepatic lectin H1; HL 1; Hs.12056
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, IP, FCM
基因名称
ASGR1
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human ASGR1 recombinant protein (Position: Y5-L291).
计算分子量
33 kDa
实际分子量
38 kDa
成分
500 ug/ml antibody with PBS,0.02% NaN3, 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the more abundant major subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Uniprot ID
P07306  
文献引用格式
ASGR1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A05376-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
基因名全称
asialoglycoprotein receptor 1
蛋白名全称
Asialoglycoprotein receptor 1
推荐稀释比
Western blot (WB):1:1000-5000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
ImmunoPrecipitation (IP):1:50
Flow Cytometry (FCM):1-3 μg/1x106 cells
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-ASGR1 Antibody

    筛选器: All WB IHC ICC/IF IP FCM

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