Anti-MMD Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of MMD using anti-MMD antibody (A06022). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human SH-SY5Y whole cell lysates,
  Lane 3: human Caco-2 whole cell lysates,
  Lane 4: human U2OS whole cell lysates,
  Lane 5: rat C6 whole cell lysates,
  Lane 6: rat RH35 whole cell lysates,
  Lane 7: mouse Neuro-2a whole cell lysates,
  Lane 8: mouse Hepa1-6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MMD antigen affinity purified polyclonal antibody (A06022) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MMD at approximately 28 kDa. The expected band size for MMD is at 28 kDa.

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  Flow Cytometry analysis of SH-SY5Y cells using anti-MMD antibody (A06022).
  Overlay histogram showing SH-SY5Y cells stained with A06022 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MMD Antibody (A06022) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  Western blot analysis of MMD using anti-MMD antibody (A06022). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human SH-SY5Y whole cell lysates,
  Lane 3: human Caco-2 whole cell lysates,
  Lane 4: human U2OS whole cell lysates,
  Lane 5: rat C6 whole cell lysates,
  Lane 6: rat RH35 whole cell lysates,
  Lane 7: mouse Neuro-2a whole cell lysates,
  Lane 8: mouse Hepa1-6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MMD antigen affinity purified polyclonal antibody (A06022) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MMD at approximately 28 kDa. The expected band size for MMD is at 28 kDa.

• 

  Flow Cytometry analysis of SH-SY5Y cells using anti-MMD antibody (A06022).
  Overlay histogram showing SH-SY5Y cells stained with A06022 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MMD Antibody (A06022) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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