Anti-BLVRB Antibody

筛选器： All WB ICC/IF FCM ELISA

• 

  Western blot analysis of anti-BLVRB antibody (A08072-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human K562 whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human A549 whole cell lysates,
  Lane 4: rat liver tissue lysates,
  Lane 5: rat RH35 whole cell lysates,
  Lane 6: mouse HEPA1-6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BLVRB antigen affinity purified polyclonal antibody (A08072-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BLVRB at approximately 22 kDa. The expected band size for BLVRB is at 22-27 kDa.

• 

  ICC/IF analysis of BLVRB using anti-BLVRB antibody (A08072-2).
  SSB was detected in an immunocytochemical section of A549 cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of MCF-7 cells using anti-BLVRB antibody (A08072-2).
  Overlay histogram showing MCF-7 cells stained with A08072-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BLVRB Antibody (A08072-2, 1:100). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  Western blot analysis of anti-BLVRB antibody (A08072-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human K562 whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human A549 whole cell lysates,
  Lane 4: rat liver tissue lysates,
  Lane 5: rat RH35 whole cell lysates,
  Lane 6: mouse HEPA1-6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BLVRB antigen affinity purified polyclonal antibody (A08072-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BLVRB at approximately 22 kDa. The expected band size for BLVRB is at 22-27 kDa.

• 

  ICC/IF analysis of BLVRB using anti-BLVRB antibody (A08072-2).
  SSB was detected in an immunocytochemical section of A549 cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of MCF-7 cells using anti-BLVRB antibody (A08072-2).
  Overlay histogram showing MCF-7 cells stained with A08072-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BLVRB Antibody (A08072-2, 1:100). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

•