Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of PACS2 using anti-PACS2 antibody (A08193-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PACS2 antigen affinity purified polyclonal antibody (A08193-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PACS2 at approximately 130 kDa. The expected band size for PACS2 is at 98 kDa.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of human testis cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of PACS2 using anti-PACS2 antibody (A08193-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PACS2 antigen affinity purified polyclonal antibody (A08193-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PACS2 at approximately 130 kDa. The expected band size for PACS2 is at 98 kDa.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of human testis cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
PACS2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.