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Anti-PACS2-Specific Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ICC/IF ELISA

A08193-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of PACS2 using anti-PACS2 antibody (A08193-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human RT4 whole cell lysates,
    Lane 2: human MCF-7 whole cell lysates,
    Lane 3: human U251 whole cell lysates,
    Lane 4: rat brain tissue lysates,
    Lane 5: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PACS2 antigen affinity purified polyclonal antibody (A08193-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PACS2 at approximately 130 kDa. The expected band size for PACS2 is at 98 kDa.

    all(9)
  • IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
    PACS2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)
  • IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
    PACS2 was detected in a paraffin-embedded section of human testis cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)
  • IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
    PACS2 was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)
  • IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
    PACS2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)
  • IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
    PACS2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)
  • IHC analysis of PACS2 using anti-PACS2 antibody (A08193-1).
    PACS2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PACS2 Antibody (A08193-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(9)

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产品简介>

产品名称
Anti-PACS2-Specific Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
KIAA0602; PACS 2; PACS1 like protein; PACS1L; PACS2; PACS-2; PACS2-Specific
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, ELISA
基因名称
PACS2
宿主
Rabbit
抗体亚型
IgG
计算分子量
98 kDa
实际分子量
130 kDa
成分
500 ug/ml antibody with PBS,0.02% NaN3, 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
PACS2 (phosphofurin acidic cluster sorting protein-2), PACS1L, is an 889 amino acid protein that localizes to both the mitochondrion and the lumen of the endoplasmic reticulum (ER) and belongs to the PACS family (1,2). It is expressed in a broad range of tissues with highest expression in skeletal muscle, brain, heart, testis and pancreas (2,). PACS2 interacts with Polycystin-2 and BID and functions as a sorting protein that regulates mitochondria-ER communication and is thought to be involved in ion channel trafficking, specifically direct cluster-containing ion channels to distinct subcellular compartments (3-5).
Uniprot ID
Q86VP3  
文献引用格式
PACS2-Specific Antibody (Boster Biological Technology, Wuhan, China. Catalog#A08193-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
基因名全称
Phosphofurin acidic cluster sorting protein 2
蛋白名全称
Phosphofurin acidic cluster sorting protein 2
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-PACS2-Specific Antibody

    筛选器: All WB IHC ICC/IF ELISA

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