Anti-H2AFY2/MACROH2A2 Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM ELISA

A09931-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of H2AFY2/MACROH2A2 using anti-H2AFY2/MACROH2A2 antibody (A09931-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-H2AFY2/MACROH2A2 antigen affinity purified polyclonal antibody (A09931-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for H2AFY2/MACROH2A2 at approximately 40 kDa. The expected band size for H2AFY2/MACROH2A2 is at 40 kDa.
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IHC analysis of H2AFY2/MACROH2A2 using anti-H2AFY2/MACROH2A2 antibody (A09931-1) .
H2AFY2/MACROH2A2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-H2AFY2/MACROH2A2 Antibody (A09931-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of H2AFY2/MACROH2A2 using anti-H2AFY2/MACROH2A2 antibody (A09931-1) .
H2AFY2/MACROH2A2 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-H2AFY2/MACROH2A2 Antibody (A09931-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of H2AFY2/MACROH2A2 using anti-H2AFY2/MACROH2A2 antibody (A09931-1) .
H2AFY2/MACROH2A2 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. The tissue section was incubated with rabbit anti-H2AFY2/MACROH2A2 Antibody (A09931-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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ICC/IF analysis of H2AFY2/MACROH2A2 using anti-H2AFY2/MACROH2A2 antibody (A09931-1) and anti-Beta Tubulin antibody (M01857-3).
H2AFY2/MACROH2A2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-H2AFY2/MACROH2A2 Antibody (A09931-1) at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
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Flow Cytometry analysis of PC-3 cells using anti-H2AFY2/MACROH2A2 antibody (A09931-1).
Overlay histogram showing PC-3 cells stained with A09931-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-H2AFY2/MACROH2A2 Antibody (A09931-1, 1:100). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-H2AFY2/MACROH2A2 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Core histone macro H2A.2; H2A histone family; member Y2; H2AFY2; histone; Histone macroH2A2; macroH2A2; mH2A2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM, ELISA

基因名称

MACROH2A2

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human H2AFY2/MACROH2A2 recombinant protein (Position: D181-D340). Human MACROH2A2 shares 99.4% amino acid (aa) sequence identity with mouse MACROH2A2.

计算分子量

40 kDa

实际分子量

40 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Core histone macro-H2A.2 is a protein that in humans is encoded by the H2AFY2 gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and may participate in stable X chromosome inactivation.

Uniprot ID

Q9P0M6

Gene ID

55506

文献引用格式

H2AFY2/MACROH2A2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A09931-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

macroH2A.2 histone

蛋白名全称

Core histone macro-H2A.2

实验方案>

实验步骤

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