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Anti-C10/CCL6 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ELISA(Cap)

A30384

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of C10/CCL6 using anti-C10/CCL6 antibody (A30384).
    Lane 1: recombinant mouse CCL6 protein 1ng.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C10/CCL6 antigen affinity purified polyclonal antibody (A30384) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for C10/CCL6 at approximately 13 kDa.

    all(3)
  • IHC analysis of C10/CCL6 using anti-C10/CCL6 antibody (A30384).
    C10/CCL6 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-C10/CCL6 Antibody (A30384) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(3)

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产品名称
Anti-C10/CCL6 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
chemokine (C-C motif) ligand 6; MRP; MRP-1; Scy; Scya6; c10; CCL6
产品类型
Polyclonal
检验物种
mouse, rat
应用范围
WB, IHC, ELISA(Cap)
基因名称
CCL6
宿主
Rabbit
抗体亚型
IgG
免疫原
E. coli-derived mouse CCL6 recombinant protein (Position: G22-A116).
计算分子量
13 kDa
实际分子量
13 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Chemokine (C-C motif) ligand 6 (CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced.CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
Uniprot ID
P27784  
RRID
文献引用格式
C10/CCL6 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A30384)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
ELISA(Cap):1:50-1:200
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-C10/CCL6 Antibody

    筛选器: All WB IHC ELISA(Cap)

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