Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
ELISA(Cap): | 1:50-1:200 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of C10/CCL6 using anti-C10/CCL6 antibody (A30384).
Lane 1: recombinant mouse CCL6 protein 1ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C10/CCL6 antigen affinity purified polyclonal antibody (A30384) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for C10/CCL6 at approximately 13 kDa.
IHC analysis of C10/CCL6 using anti-C10/CCL6 antibody (A30384).
C10/CCL6 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-C10/CCL6 Antibody (A30384) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of C10/CCL6 using anti-C10/CCL6 antibody (A30384).
Lane 1: recombinant mouse CCL6 protein 1ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C10/CCL6 antigen affinity purified polyclonal antibody (A30384) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for C10/CCL6 at approximately 13 kDa.
IHC analysis of C10/CCL6 using anti-C10/CCL6 antibody (A30384).
C10/CCL6 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-C10/CCL6 Antibody (A30384) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.