| Western blot (WB): | 1:500-2000 |
| ELISA: | 1:100-1000 |

Western blot analysis of GRK3 using anti-GRK3 antibody (A32254-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HL-60 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRK3 antigen affinity purified polyclonal antibody (A32254-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRK3 at approximately 80 kDa. The expected band size for GRK3 is at 80 kDa.

Western blot analysis of GRK3 using anti-GRK3 antibody (A32254-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HL-60 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRK3 antigen affinity purified polyclonal antibody (A32254-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRK3 at approximately 80 kDa. The expected band size for GRK3 is at 80 kDa.


