Anti-Zebrafish ACC1/ACACA Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF

AZA0A8M6YZ52

50μl ￥1380 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ACC1/ACACA using anti-ACC1/ACACA antibody (AZA0A8M6YZ52). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACC1/ACACA antigen affinity purified polyclonal antibody (AZA0A8M6YZ52) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACC1/ACACA at approximately 269 kDa. The expected band size for ACC1/ACACA is at 269 kDa.
all(4)
IHC analysis of ACC1/ACACA using anti-ACC1/ACACA antibody (AZA0A8M6YZ52).
ACC1/ACACA was detected in a paraffin-embedded section of zebrafish liver tissue. The tissue section was incubated with rabbit anti-ACC1/ACACA Antibody (AZA0A8M6YZ52) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of ACC1/ACACA using anti-ACC1/ACACA antibody (AZA0A8M6YZ52).
ACC1/ACACA was detected in a paraffin-embedded section of zebrafish ovary tissue. The tissue section was incubated with rabbit anti-ACC1/ACACA Antibody (AZA0A8M6YZ52) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IF analysis of ACC1/ACACA using anti-ACC1/ACACA antibody (AZA0A8M6YZ52).
ACC1/ACACA was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-ACC1/ACACA Antibody (AZA0A8M6YZ52) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Zebrafish ACC1/ACACA Antibody

规格/价格

50μl|1380 100μl|2180 150μl|2800

指标别称

ACAC; ACACA; ACC; ACC1; ACCA; Acetyl CoA carboxylase 1

产品类型

Polyclonal

检验物种

zebrafish

应用范围

WB, IHC, IF

基因名称

ACACA

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived Zebrafish ACC1/ACACA recombinant protein (Position: I799-H1324).

计算分子量

269 kDa

实际分子量

269 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Predicted to enable acetyl-CoA carboxylase activity. Acts upstream of or within response to (R)-carnitine. Predicted to be located in cytoplasm. Predicted to be active in mitochondrion. Is expressed in female organism; liver; male organism; subcutaneous fat; and visceral fat. Orthologous to human ACACA (acetyl-CoA carboxylase alpha).

Uniprot ID

A0A8M6YZ52

Gene ID

559403

文献引用格式

Zebrafish ACC1/ACACA Antibody (Boster Biological Technology, Wuhan, China. Catalog#AZA0A8M6YZ52)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

acetyl-CoA carboxylase alpha

蛋白名全称

acetyl-CoA carboxylase

实验方案>

实验步骤

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