Anti-Zebrafish AFF4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

AZA0A8N7TEI3

50μl ￥1380 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of AFF4 using anti-AFF4 antibody (AZA0A8N7TEI3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AFF4 antigen affinity purified polyclonal antibody (AZA0A8N7TEI3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AFF4 at approximately 170 kDa. The expected band size for AFF4 is at 127 kDa.
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产品简介>

产品名称

Anti-Zebrafish AFF4 Antibody

规格/价格

50μl/1380 100μl/2180 150μl/2800

指标别称

AF4/FMR2 family member 4; AF4/FMR2 family; member 4; AF5Q31; AFF4; MCEF; Protein AF 5q31

产品类型

Polyclonal

检验物种

zebrafish

应用范围

基因名称

AFF4

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived zebrafish AFF4 recombinant protein (Position: M1-E60).

计算分子量

127 kDa

实际分子量

170 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The AFF4 gene encodes a scaffold protein that functions as a core component of the super elongation complex (SEC), which is involved in transcriptional regulation during embryogenesis. The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. This gene is mapped to chromosome 5q31.

Uniprot ID

A0A8N7TEI3

Gene ID

556421

文献引用格式

Zebrafish AFF4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#AZA0A8N7TEI3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

AF4/FMR2 family, member 4

蛋白名全称

AF4/FMR2 family member 4 isoform X3

实验方案>

实验步骤

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