Anti-Zebrafish ACADM Antibody

Rabbit polyclonal antibody

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筛选器： All WB

AZA2CG95

50μl ￥1380 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ACADM using anti-ACADM antibody (AZA2CG95). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: female zebrafish viscera tissue lysates,
Lane 3: male zebrafish viscera tissue lysates,
Lane 4: whole zebrafish tissue lysates,
Lane 5: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACADM antigen affinity purified polyclonal antibody (AZA2CG95) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACADM at approximately 43 kDa. The expected band size for ACADM is at 47 kDa.
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产品名称

Anti-Zebrafish ACADM Antibody

规格/价格

50μl/1380 100μl/2180 150μl/2800

指标别称

ACAD1; ACADM; MCAD; MCADH

产品类型

Polyclonal

检验物种

zebrafish

应用范围

基因名称

ACADM

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of zebrafish ACADM.

计算分子量

47 kDa

实际分子量

43 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ACADM (acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain) is a gene that provides instructions for making an enzyme called acyl-coenzyme A dehydrogenase that is important for breaking down (degrading) a certain group of fats called medium-chain fatty acids. This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

A2CG95

Gene ID

406283

文献引用格式

Zebrafish ACADM Antibody (Boster Biological Technology, Wuhan, China. Catalog#AZA2CG95)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

acyl-CoA dehydrogenase medium chain

蛋白名全称

Medium-chain specific acyl-CoA dehydrogenase, mitochondrial

实验方案>

实验步骤

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