Anti-Zebrafish Androgen receptor/AR Antibody

Rabbit polyclonal antibody

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筛选器： All WB

AZA4GT83

50μl ￥1380 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Androgen receptor/AR using anti-Androgen receptor/AR antibody (AZA4GT83). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Androgen receptor/AR antigen affinity purified polyclonal antibody (AZA4GT83) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Androgen receptor/AR at approximately 96 kDa. The expected band size for Androgen receptor/AR is at 96 kDa.
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产品名称

Anti-Zebrafish Androgen receptor/AR Antibody

规格/价格

50μl/1380 100μl/2180 150μl/2800

指标别称

AIS; androgen receptor; AR; DHTR; Dihydrotestosterone receptor; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM

产品类型

Polyclonal

检验物种

zebrafish

应用范围

基因名称

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived zebrafish Androgen receptor/AR recombinant protein (Position: E12-Q852)

计算分子量

96 kDa

实际分子量

96 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The AR (androgen receptor) gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The AR gene is mapped to Xq12. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

Uniprot ID

A4GT83

文献引用格式

Zebrafish Androgen receptor/AR Antibody (Boster Biological Technology, Wuhan, China. Catalog#AZA4GT83)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

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