Anti-Zebrafish AKT3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

AZD9IL79

50μl ￥1380 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of AKT3 using anti-AKT3 antibody (AZD9IL79). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: female zebrafish viscera tissue lysates,
Lane 2: male zebrafish viscera tissue lysates,
Lane 3: whole zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (AZD9IL79) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKT3 at approximately 56 kDa. The expected band size for AKT3 is at 56 kDa.
all(1)
Western blot analysis of AKT3 using anti-AKT3 antibody (AZD9IL79). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: female zebrafish viscera tissue lysates,
Lane 2: male zebrafish viscera tissue lysates,
Lane 3: whole zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (AZD9IL79) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKT3 at approximately 56 kDa. The expected band size for AKT3 is at 56 kDa.
all(1)
Western blot analysis of AKT3 using anti-AKT3 antibody (AZD9IL79). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: female zebrafish viscera tissue lysates,
Lane 2: male zebrafish viscera tissue lysates,
Lane 3: whole zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (AZD9IL79) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKT3 at approximately 56 kDa. The expected band size for AKT3 is at 56 kDa.
all(1)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Zebrafish AKT3 Antibody

规格/价格

50μl/1380 100μl/2180 150μl/2800

指标别称

AKT3; PKB GAMMA; PKBG; PRKBG; Protein kinase Akt 3; Protein kinase B gamma; RAC gamma; RAC PK gamma; STK 2

产品类型

Polyclonal

检验物种

zebrafish

应用范围

基因名称

AKT3

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived zebrafish AKT3 recombinant protein (Position: M104-L154).

计算分子量

56 kDa

实际分子量

56 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

RAC-gamma serine/threonine-protein kinase, also known as protein kinase Akt-3, is an enzyme that in humans is encoded by the AKT3 gene. This gene is mapped to 1q43-q44. The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor(PDGF), insulin, and insulin-like growth factor 1(IGF1). AKT3 plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. This gene is required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands.

Uniprot ID

D9IL79

Gene ID

560549

文献引用格式

Zebrafish AKT3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#AZD9IL79)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

AKT serine/threonine kinase 3

蛋白名全称

RAC-gamma serine/threonine-protein kinase

实验方案>

实验步骤

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