Anti-Zebrafish AGO1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

AZK4I6K9

50μl ￥1380 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of AGO1 using anti-AGO1 antibody (AZK4I6K9). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AGO1 antigen affinity purified polyclonal antibody (AZK4I6K9) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AGO1 at approximately 97 kDa. The expected band size for AGO1 is at 97 kDa.
all(1)
Western blot analysis of AGO1 using anti-AGO1 antibody (AZK4I6K9). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AGO1 antigen affinity purified polyclonal antibody (AZK4I6K9) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AGO1 at approximately 97 kDa. The expected band size for AGO1 is at 97 kDa.
all(1)
Western blot analysis of AGO1 using anti-AGO1 antibody (AZK4I6K9). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AGO1 antigen affinity purified polyclonal antibody (AZK4I6K9) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AGO1 at approximately 97 kDa. The expected band size for AGO1 is at 97 kDa.
all(1)

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产品简介>

产品名称

Anti-Zebrafish AGO1 Antibody

规格/价格

50μl/1380 100μl/2180 150μl/2800

指标别称

AGO1; Argonaute1; eIF 2C 1; EIF2C; eIF2C 1; EIF2C1; EIF2C1-Specific; GERP95; hAgo1; Protein argonaute 1; Q99

产品类型

Polyclonal

检验物种

zebrafish

应用范围

基因名称

AGO1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived zebrafish AGO1 recombinant protein (Position: E377-R410).

计算分子量

97 kDa

实际分子量

97 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.

Uniprot ID

K4I6K9

Gene ID

570775

文献引用格式

Zebrafish AGO1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#AZK4I6K9)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

argonaute RISC component 1

蛋白名全称

Protein argonaute-1

实验方案>

实验步骤

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