Western blot (WB): | 1:500-2000 |
Western blot analysis of Alpha Parvin/Actopaxin/PARVA using anti-Alpha Parvin/Actopaxin/PARVA antibody (AZQ6DRM4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: female zebrafish viscera tissue lysates,
Lane 3: male zebrafish viscera tissue lysates,
Lane 4: whole female zebrafish tissue lysates,
Lane 5: whole male zebrafish tissue lysates,
Lane 6: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alpha Parvin/Actopaxin/PARVA antigen affinity purified polyclonal antibody (AZQ6DRM4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alpha Parvin/Actopaxin/PARVA at approximately 42 kDa. The expected band size for Alpha Parvin/Actopaxin/PARVA is at 42 kDa.
Western blot analysis of Alpha Parvin/Actopaxin/PARVA using anti-Alpha Parvin/Actopaxin/PARVA antibody (AZQ6DRM4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: female zebrafish viscera tissue lysates,
Lane 3: male zebrafish viscera tissue lysates,
Lane 4: whole female zebrafish tissue lysates,
Lane 5: whole male zebrafish tissue lysates,
Lane 6: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alpha Parvin/Actopaxin/PARVA antigen affinity purified polyclonal antibody (AZQ6DRM4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alpha Parvin/Actopaxin/PARVA at approximately 42 kDa. The expected band size for Alpha Parvin/Actopaxin/PARVA is at 42 kDa.