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Anti-Zebrafish ABCE1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

AZQ6TNW3-1

  • 50μl ¥1380 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: zebrafish head tissue lysates,
    Lane 2: zebrafish embryo tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (AZQ6TNW3-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.

    all(5)
  • IHC analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3-1) .
    ABCE1 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody (AZQ6TNW3-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3-1) .
    ABCE1 was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody (AZQ6TNW3-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3-1) .
    ABCE1 was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody (AZQ6TNW3-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3-1) .
    ABCE1 was detected in a paraffin-embedded section of zebrafish retina tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody (AZQ6TNW3-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(5)

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产品名称
Anti-Zebrafish ABCE1 Antibody
规格/价格
50μl/1380 100μl/2180 150μl/2800
指标别称
ABCE1; RLI; RNASEL1; RNASELI; RNS4I; OK/SW-cl.40; ATP-binding cassette sub-family E member 1; 2'-5'-oligoadenylate-binding protein; HuHP68; RNase L inhibitor; Ribonuclease 4 inhibitor; RNS4I
产品类型
Polyclonal
检验物种
zebrafish
应用范围
WB, IHC
基因名称
ABCE1
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived zebrafish ABCE1 recombinant protein (Position: Q141-D599).
计算分子量
67 kDa
实际分子量
67 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
ATP-binding cassette sub-family E member 1 (ABCE1) also known as RNase L inhibitor (RLI) is an enzyme that in humans is encoded by the ABCE1 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene.
Uniprot ID
Q6TNW3  
Gene ID
406324  
文献引用格式
Zebrafish ABCE1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#AZQ6TNW3-1)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
ATP binding cassette subfamily E member 1
蛋白名全称
ATP-binding cassette sub-family E member 1
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Zebrafish ABCE1 Antibody

    筛选器: All WB IHC

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