| Western blot (WB): | 1:500-2000 |

Western blot analysis of MYOD1 using anti-MYOD1 antibody (AZQ90477). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
Lane 2: whole female zebrafish tissue lysates.
Lane 3: whole male zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MYOD1 antigen affinity purified polyclonal antibody (AZQ90477) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MYOD1 at approximately 40 kDa. The expected band size for MYOD1 is at 35 kDa.

Western blot analysis of MYOD1 using anti-MYOD1 antibody (AZQ90477). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
Lane 2: whole female zebrafish tissue lysates.
Lane 3: whole male zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MYOD1 antigen affinity purified polyclonal antibody (AZQ90477) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MYOD1 at approximately 40 kDa. The expected band size for MYOD1 is at 35 kDa.
