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Anti-Annexin A2/ANXA2 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA0641

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of Annexin A2/ANXA2 using anti-Annexin A2/ANXA2 antibody (BA0641). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat testis tissue lysates,
    Lane 2: rat lung tissue lysates,
    Lane 3: rat ovary tissue lysates,
    Lane 4: human MCF-7 whole cell lysates,
    Lane 5: human SMMC whole cell lysates,
    Lane 6: human A549 whole cell lysates,
    Lane 7: human Jurkat whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Annexin A2/ANXA2 antigen affinity purified polyclonal antibody (BA0641) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Annexin A2/ANXA2 at approximately 36 kDa. The expected band size for Annexin A2/ANXA2 is at 39 kDa.

    all(3)
  • Western blot analysis of Annexin A2/ANXA2 using anti-Annexin A2/ANXA2 antibody (BA0641). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: mouse spleen tissue lysates,
    Lane 2: mouse NIH/3T3 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Annexin A2/ANXA2 antigen affinity purified polyclonal antibody (BA0641) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Annexin A2/ANXA2 at approximately 36 kDa. The expected band size for Annexin A2/ANXA2 is at 39 kDa.

    all(3)
  • IHC analysis of Annexin A2/ANXA2 using anti-Annexin A2/ANXA2 antibody (BA0641) .
    Annexin A2/ANXA2 was detected in a paraffin-embedded section of human intestinal tissue. The tissue section was incubated with rabbit anti-Annexin A2/ANXA2 Antibody (BA0641) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(3)

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产品名称
Anti-Annexin A2/ANXA2 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Annexin 2; annexin A2; Annexin II; ANX2; ANX2L4; ANXA2; CAL1H; Calpactin 1 heavy chain; Calpactin I heavy chain; Chromobindin 8; LIP2; Lipocortin II; LPC2; LPC2D; P36; PAP IV; Protein I
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
ANXA2
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence in the middle region of human Annexin A2, different from the rat sequence by one amino acid.
计算分子量
39 kDa
实际分子量
36 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. The ANXA2 gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. This protein is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. Richard et al.(1994) presented an integration of the physical, expression, and genetic maps of human chromosome 15. They placed the ANXA2 gene in their region IV, i.e., 15q21-q22, thus confirming the previous localization.
Uniprot ID
P07355  
文献引用格式
Annexin A2/ANXA2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0641)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
annexin A2
蛋白名全称
Annexin A2
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Annexin A2/ANXA2 Antibody

    筛选器: All WB IHC

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