Anti-APC Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC

BA0645

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of APC using anti-APC antibody (BA0645). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: Colo320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-APC antigen affinity purified polyclonal antibody (BA0645) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for APC at approximately 160,312 kDa. The expected band size for APC is at 312 kDa.
all(3)
IHC analysis of APC using anti-APC antibody (BA0645).
APC was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-APC Antibody (BA0645) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of APC using anti-APC antibody (BA0645).
APC was detected in a paraffin-embedded section of intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-APC Antibody (BA0645) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-APC Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

adenomatous polyposis coli; APC; BTPS2; Deleted in polyposis 2.5; DP2; DP2.5; DP3; GS; Protein APC

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

APC

宿主

Rabbit

抗体亚型

IgG

免疫原

Polypeptide

计算分子量

312 kDa

实际分子量

160,312 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]

Uniprot ID

P25054

文献引用格式

APC Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0645)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

引用文献(0)>

清除