Anti-APC Antibody (Clone#FAD-1)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP

BM4480

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-APC antibody (BM4480). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-APC antigen affinity purified monoclonal antibody (BM4480) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for APC at approximately 160 kDa. The expected band size for APC is at 312 kDa.
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IHC analysis of APC using anti-APC antibody (BM4480) .
APC was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-APC Antibody (BM4480) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of APC using anti-APC antibody (BM4480) .
APC was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-APC Antibody (BM4480) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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产品简介>

产品名称

Anti-APC Antibody (Clone#FAD-1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

adenomatous polyposis coli; APC; BTPS2; Deleted in polyposis 2.5; DP2; DP2.5; DP3; GS; Protein APC

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

APC

克隆号

FAD-1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human APC Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF) -induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.

计算分子量

312 kDa

实际分子量

160,312 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]

Uniprot ID

P25054

文献引用格式

APC Antibody (Clone#FAD-1) (Boster Biological Technology, Wuhan, China. Catalog#BM4480)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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