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Anti-CTGF/CCN2 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA0752

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of CTGF/CCN2 using anti-CTGF/CCN2 antibody (BA0752). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: HepG2 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CTGF/CCN2 antigen affinity purified polyclonal antibody (BA0752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CTGF/CCN2 at approximately 38 kDa. The expected band size for CTGF/CCN2 is at 38 kDa.

    all(2)
  • IHC analysis of CTGF/CCN2 using anti-CTGF/CCN2 antibody (BA0752).
    CTGF/CCN2 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CTGF/CCN2 Antibody (BA0752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(2)

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产品简介>

产品名称
Anti-CTGF/CCN2 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CCN family member 2; CCN2; CTGF; HCS24; IBP 8; IGF binding protein 8; IGFBP 8; IGFBP8; NOV2
产品类型
Polyclonal
检验物种
human, mouse, rat, rabbit
应用范围
WB, IHC
基因名称
CCN2
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human CTGF recombinant protein (Position: R58-A349). Human CTGF shares 95.5% and 95.9% amino acid (aa) sequence identity with mouse and rat CTGF, respectively.
计算分子量
38 kDa
实际分子量
38 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
CTGF, also known as CCN2 or connective tissue growth factor, is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins (see also CCN intercellular signaling protein). CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. And the encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
Uniprot ID
P29279  
文献引用格式
CTGF/CCN2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0752)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-CTGF/CCN2 Antibody

    筛选器: All WB IHC

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