Anti-GRP94/HSP90B1 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF

BA0930

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of GRP94/HSP90B1 using anti-GRP94/HSP90B1 antibody (BA0930). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRP94/HSP90B1 antigen affinity purified polyclonal antibody (BA0930) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRP94/HSP90B1 at approximately 100 kDa. The expected band size for GRP94/HSP90B1 is at 92 kDa.
all(4)
IHC analysis of GRP94/HSP90B1 using anti-GRP94/HSP90B1 antibody (BA0930).
GRP94/HSP90B1 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-GRP94/HSP90B1 Antibody (BA0930) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of GRP94/HSP90B1 using anti-GRP94/HSP90B1 antibody (BA0930).
GRP94/HSP90B1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GRP94/HSP90B1 Antibody (BA0930) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
ICC analysis of GRP94/HSP90B1 using anti- GRP94/HSP90B1 antibody (BA0930).
GRP94/HSP90B1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-GRP94/HSP90B1 Antibody (BA0930) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-GRP94/HSP90B1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell and organelle markers; ECGP; endoplasmic reticulum marker; Endoplasmin; GP96; gp96 homolog; GRP 94; GRP94; HSP90B1; TRA1; Tumor rejection antigen 1

产品类型

Polyclonal

检验物种

human, rat

应用范围

WB, IHC, ICC/IF

基因名称

HSP90B1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human GRP94, identical to the related mouse and rat sequences.

计算分子量

92 kDa

实际分子量

100 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Heat shock protein 90kDa beta member 1 (HSP90B1), known also as endoplasmin, GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation.

Uniprot ID

P14625

文献引用格式

GRP94/HSP90B1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0930)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

heat shock protein 90kDa beta(Grp94), member 1

蛋白名全称

Endoplasmin

实验方案>

实验步骤

引用文献(0)>

清除