Anti-MMP16 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA1280

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of MMP16 using anti-MMP16 antibody (BA1280). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO-320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MMP16 antigen affinity purified polyclonal antibody (BA1280) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MMP16 at approximately 69 kDa or 53 kDa. The expected band size for MMP16 is at 70 kDa.
all(4)
IHC analysis of MMP16 using anti-MMP16 antibody (BA1280).
MMP16 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MMP16 Antibody (BA1280) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of MMP16 using anti-MMP16 antibody (BA1280).
MMP16 was detected in frozen section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MMP16 Antibody (BA1280) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of MMP16 using anti-MMP16 antibody (BA1280).
MMP16 was detected in frozen section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MMP16 Antibody (BA1280) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-MMP16 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

matrix metallopeptidase 16; C8orf57; MMP-X2; MT-MMP2; MT-MMP3; MT3-MMP; MMP16

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

MMP16

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human MMP16, identical to the related rat and mouse sequences.

计算分子量

70 kDa

实际分子量

69 kDa or 53 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The matrix metalloproteinase 16(MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.

Uniprot ID

P51512

文献引用格式

MMP16 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1280)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

matrix metallopeptidase 16(membrane-inserted)

蛋白名全称

Matrix metalloproteinase-16(MMP-16)

实验方案>

实验步骤

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