Anti-AQP3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BA1559

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of AQP3 using anti-AQP3 antibody (BA1559). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AQP3 antigen affinity purified polyclonal antibody (BA1559) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AQP3 at approximately 32 kDa. The expected band size for AQP3 is at 32 kDa.
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IHC analysis of AQP3 using anti-AQP3 antibody (BA1559).
AQP3 was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-AQP3 Antibody (BA1559) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of AQP3 using anti-AQP3 antibody (BA1559).
AQP3 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-AQP3 Antibody (BA1559) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of AQP3 using anti-AQP3 antibody (BA1559).
AQP3 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-AQP3 Antibody (BA1559) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of AQP3 using anti-AQP3 antibody (BA1559).
AQP3 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-AQP3 Antibody (BA1559) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
ICC/IF analysis of AQP3 using anti-AQP3 antibody (BA1559).
AQP3 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-AQP3 Antibody (BA1559) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of RT4 cells using anti-AQP3 antibody (BA1559).
Overlay histogram showing RT4 cells stained with BA1559 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP3 Antibody (BA1559) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(7)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-AQP3 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

aquaporin 3; AQP-3; GIL; AQP3

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

AQP3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3, different from the related rat and mouse sequences by one amino acid.

计算分子量

32 kDa

实际分子量

32 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Uniprot ID

Q92482

文献引用格式

AQP3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1559)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

aquaporin 3(Gill blood group)

蛋白名全称

Aquaporin-3

实验方案>

实验步骤

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