Anti-Calpain 2/CAPN2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF FCM

BA1575

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Calpain 2/CAPN2 using anti-Calpain 2/CAPN2 antibody (BA1575). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Calpain 2/CAPN2 antigen affinity purified polyclonal antibody (BA1575) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calpain 2/CAPN2 at approximately 80 kDa. The expected band size for Calpain 2/CAPN2 is at 80 kDa.
all(3)
IF analysis of Calpain 2/CAPN2 using anti-Calpain 2/CAPN2 antibody (BA1575).
Calpain 2/CAPN2 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Calpain 2/CAPN2 Antibody (BA1575) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(3)
Flow Cytometry analysis of U2OS cells using anti-Calpain 2/CAPN2 antibody (BA1575).
Overlay histogram showing U2OS cells stained with BA1575 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpain 2/CAPN2 Antibody (BA1575) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(3)

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产品名称

Anti-Calpain 2/CAPN2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Calpain 2; Calpain 2 catalytic subunit; Calpain 2 large subunit; Calpain large polypeptide L2; Calpain M type; CANP 2; CANP2; CANPL2; CANPml; CAPN2; M calpain; mCANP; Millimolar calpain

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, FCM

基因名称

CAPN2

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5% and 92.5% amino acid (aa) sequence identity with mouse and rat Calpain 2, respectively.

计算分子量

80 kDa

实际分子量

80 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Calpain-2 catalytic subunit is a protein that in humans is encoded by the CAPN2 gene. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported.

Uniprot ID

P17655

文献引用格式

Calpain 2/CAPN2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1575)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

calpain 2

蛋白名全称

Calpain-2 catalytic subunit

实验方案>

实验步骤

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