Anti-Calpain 2/CAPN2 Antibody (Clone#OTI3G1)

Mouse monoclonal antibody

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筛选器： All IHC WB

M03492-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CAPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M03492-3)
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and CAPN2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-CAPN2 monoclonal antibody M03492-3, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody ([M01263-6]) as a loading control.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN2(Cat# M03492-3).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CAPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M03492-3)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CAPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M03492-3)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CAPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M03492-3)
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:500).
all(7)

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产品名称

Anti-Calpain 2/CAPN2 Antibody (Clone#OTI3G1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Calpain 2; Calpain 2 catalytic subunit; Calpain 2 large subunit; Calpain large polypeptide L2; Calpain M type; CANP 2; CANP2; CANPL2; CANPml; CAPN2; M calpain; mCANP; Millimolar calpain

产品类型

Monoclonal

检验物种

human, mouse, rat, dog

应用范围

IHC, WB

基因名称

CAPN2

克隆号

OTI3G1

宿主

Mouse

抗体亚型

IgG2a

免疫原

Full length human recombinant protein of human CAPN2(NP_001739) produced in HEK293T cell.

计算分子量

80 kDa

实际分子量

80 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot ID

P17655

文献引用格式

Calpain 2/CAPN2 Antibody (Clone#OTI3G1) (Boster Biological Technology, Wuhan, China. Catalog#M03492-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

calpain 2

蛋白名全称

Calpain-2 catalytic subunit

实验方案>

实验步骤

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