Anti-MCP-1/CCL2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA1843-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of MCP-1/CCL2 using anti-MCP-1/CCL2 antibody (BA1843-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: MCF-7 whole cell lysates,
Lane 3: Raji whole cell lysates,
Lane 4: Colo320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MCP-1/CCL2 antigen affinity purified polyclonal antibody (BA1843-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MCP-1/CCL2 at approximately 25 kDa. The expected band size for MCP-1/CCL2 is at 11 kDa.
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产品简介>

产品名称

Anti-MCP-1/CCL2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

C C motif chemokine 2; CCL2; chemokine (C C motif) ligand 2; GDCF 2; HC11; HSMCR30; MCAF; MCP 1; MCP1; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2; SMC CF

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

CCL2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human MCP1, different from the mouse and rat sequences by two amino acids.

计算分子量

11 kDa

实际分子量

25 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine(chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

Uniprot ID

P13500

文献引用格式

MCP-1/CCL2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1843-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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