Anti-MT-CO1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

BA2149-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-MT-CO1 antibody (BA2149-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HeLa mitochondria lysates at 20ug,
Lane 2: human HeLa whole cell lysates at 20ug,
Lane 3: human Caco-2 whole cell lysates at 50ug,
Lane 4: rat heart tissue lysates at 50ug.
Lane 5: mouse heart tissue lysates at 50ug.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MT-CO1 antigen affinity purified polyclonal antibody (BA2149-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MT-CO1 at approximately 37 kDa. The expected band size for MT-CO1 is at 57 kDa.
all(3)
IHC analysis of MT-CO1 using anti-MT-CO1 antibody (BA2149-2).
MT-CO1 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
ICC analysis of MT-CO1 using anti-MT-CO1 antibody (BA2149-2).
MT-CO1 was detected in an immunocytochemical section of C6 cells. The section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-MT-CO1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

mitochondrially encoded cytochrome c oxidase; COI; MTCO1; COX1

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, ICC/IF

基因名称

MT-CO1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human MTCO1.

计算分子量

57 kDa

实际分子量

37 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cytochrome c oxidase subunit I(CO1 or MTCO1) is 1 of 3 mitochondrial DNA(mtDNA) encoded subunits(MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III(MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al.(2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase(COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

Uniprot ID

P00395

文献引用格式

MT-CO1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2149-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

mitochondrially encoded cytochrome c oxidase I

蛋白名全称

Cytochrome c oxidase subunit 1

实验方案>

实验步骤

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