Anti-SOCS3 Antibody
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Rabbit polyclonal antibody

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筛选器： All WB IHC

BA2375

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SOCS3 using anti-SOCS3 antibody (BA2375). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOCS3 antigen affinity purified polyclonal antibody (BA2375) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOCS3 at approximately 28-30 kDa. The expected band size for SOCS3 is at 25 kDa.
all(2)
IHC analysis of SOCS3 using anti-SOCS3 antibody (BA2375).
SOCS3 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SOCS3 Antibody (BA2375) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品名称

Anti-SOCS3 Antibody

规格/价格

50μl|1280元
100μl|2180元
150μl|2800元

指标别称

ATOD4; CIS 3; CIS3; Cish3; SOCS 3; SOCS3; SOCS-3; SSI 3; SSI3; STAT induced STAT inhibitor 3

产品类型

Polyclonal

检验物种

human, mouse

应用范围

WB, IHC

基因名称

SOCS3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human SOCS3, identical to the related mouse sequence, different from the related rat sequence by one amino acid.

计算分子量

25 kDa

实际分子量

28-30 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

SOCS3(Suppressor of cytokine signaling 3) is a protein that in humans is encoded by the SOCS3 gene. SOCS3 is transiently expressed by multiple cell lineages within the immune system and functions predominantly as a negative regulator of cytokines that activate the JAK-STAT3 pathway. This gene encodes a member of the STAT-induced STAT inhibitor(SSI), also known as suppressor of cytokine signaling(SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon(IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. For signaling of IL-6, Epo, GCSF and Leptin, binding of SOCS3 to the respective cytokine receptor has been found to be crucial for the inhibitory function of SOCS3. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.

Uniprot ID

O14543

文献引用格式

SOCS3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2375)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

suppressor of cytokine signaling 3

蛋白名全称

Suppressor of cytokine signaling 3(SOCS-3)

实验方案>

实验步骤

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