Anti-Hamartin/TSC1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA2879

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (BA2879). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat skeletal muscle tissue lysates,
Lane 2: Rat heart tissue lysates,
Lane 3: Rat brain tissue lysates,
Lane 4: Rat lung tissue lysates,
Lane 5: 293T whole cell lysates,
Lane 6: HELA whole cell lysates,
Lane 7: HT1080 whole cell lysates,
Lane 8: SMMC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Hamartin/TSC1 antigen affinity purified polyclonal antibody (BA2879) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Hamartin/TSC1 at approximately 150 kDa. The expected band size for Hamartin/TSC1 is at 130 kDa.
all(3)
IHC analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (BA2879).
Hamartin/TSC1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Hamartin/TSC1 Antibody (BA2879) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (BA2879).
Hamartin/TSC1 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Hamartin/TSC1 Antibody (BA2879) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-Hamartin/TSC1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Hamartin; KIAA0243; LAM; TSC; TSC1; tuberous sclerosis 1; Tuberous sclerosis 1 protein

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

TSC1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Hamartin, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

计算分子量

130 kDa

实际分子量

150 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

TSC1(Tuberous Sclerosis 1), also called HAMARTIN or TSC, is a human protein and gene. As part of a comprehensive strategy to identify the gene mutant in tuberous sclerosis-1, van Slegtenhorst et al.(1997) developed an overlapping contig of clones from the 1.4-Mb TSC1 region on chromosome 9. Benvenuto et al.(2000) showed that overexpression of the TSC1 gene in rat fibroblasts inhibits growth and causes changes in cell morphology. Van Slegtenhorst et al.(1998) showed that hamartin and tuberin associate physically in vivo, however, and that the interaction is mediated by predicted coiled-coil domains.

Uniprot ID

Q92574

文献引用格式

Hamartin/TSC1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2879)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

tuberous sclerosis 1

蛋白名全称

Hamartin

实验方案>

实验步骤

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