Anti-DNA-PKcs/PRKDC Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA2908-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-DNA-PKcs/PRKDC antibody (BA2908-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HEK293 whole cell lysates .
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DNA-PKcs/PRKDC antigen affinity purified polyclonal antibody (BA2908-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DNA-PKcs/PRKDC at approximately 460 kDa. The expected band size for DNA-PKcs/PRKDC is at 469 kDa.
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产品名称

Anti-DNA-PKcs/PRKDC Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

DNA PK catalytic subunit; DNAPK; DNA-PKcs; DNPK1; HYRC; HYRC1; p350; p460; PRKDC; XRCC7

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

PRKDC

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human DNA PKcs.

计算分子量

469 kDa

实际分子量

469 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PRKDC(Protein Kinase DNA-Activated Catalytic Subunit), also called DNAPK, HYRC1, p350 or DNPK1, is an enzyme that in humans is encoded by the PRKDC gene. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Satoh et al.(1997) mapped the MCM4 gene to 8q11.2 by FISH. Based on the close proximity of the PRKDC and MCM4 genes, it was assumed that the PRKDC gene also maps to this location. Anderson and Lees-Miller(1992) noted that DNA-PK had been shown in vitro to phosphorylate several transcription factors, suggesting that it functions in cell homeostasis by modulating transcription. Daniel et al.(1999) demonstrated that the PRKDC protein participates in retroviral DNA integration, which is catalyzed by the viral protein integrase.

Uniprot ID

P78527

文献引用格式

DNA-PKcs/PRKDC Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2908-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

protein kinase, DNA-activated, catalytic polypeptide

蛋白名全称

DNA-dependent protein kinase catalytic subunit

实验方案>

实验步骤

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