Anti-VGF Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA3095-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of VGF using anti-VGF antibody (BA3095-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue,
Lane 2: U87 whole cell lysates,
Lane 3: U87 whole cell lysates,
Lane 4: SHG whole cell lysates,
Lane 5: NEURO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VGF antigen affinity purified polyclonal antibody (BA3095-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VGF at approximately 67 kDa. The expected band size for VGF is at 67 kDa.
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产品简介>

产品名称

Anti-VGF Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Neurosecretory protein VGF; VGF

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

VGF

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human VGF, different from the related rat and mouse sequences by one amino acid.

计算分子量

67 kDa

实际分子量

67 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

VGF(VGF, Nerve Growth Factor-Inducible), is a protein and neuropeptide that may play a role in regulating energy homeostasis, metabolism. Canu et al.(1997) noted that rat Vgf encodes a predicted 70-kD polypeptide that shares similarities with the secretogranin/chromogranin family and is found in the secretory granules of subsets of neurons and endocrine cells. By fluorescence in situ hybridization, Canu et al.(1997) assigned the VGF gene to 7q22. Canu et al.(1997) demonstrated that the single-copy human VGF gene spans 6 kb of genomic DNA and contains 2 exons. The entire VGF protein is encoded by exon 2, while exon 1 contains only 5-prime untranslated sequence. The structural organization of the human gene is similar to that described for the rat VGF gene(Salton et al., 1991), and both the translated and the untranslated regions show a high degree of sequence homology to the rat gene.

Uniprot ID

O15240

文献引用格式

VGF Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3095-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

VGF nerve growth factor inducible

蛋白名全称

Neurosecretory protein VGF

实验方案>

实验步骤

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