Anti-NRG1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

BA3235

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of NRG1 using anti-NRG1 antibody (BA3235). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NRG1 antigen affinity purified polyclonal antibody (BA3235) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NRG1 at approximately 100,65,40 kDa. The expected band size for NRG1 is at 70,65,40,25 kDa.
all(3)
IHC analysis of NRG1 using anti-NRG1 antibody (BA3235).
NRG1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-NRG1 Antibody (BA3235) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IF analysis of NRG1 using anti-NRG1 antibody (BA3235) and anti-Beta Tubulin antibody (M01857-3).
NRG1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-NRG1 Antibody (BA3235) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Dylight550-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog#BA1133) were used as secondary antibody.
all(3)

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产品简介>

产品名称

Anti-NRG1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ARIA; GGF; GGF2; Glial growth factor; Heregulin; HGL; HRG; HRG1; HRGA; NDF; Neu differentiation factor; neuregulin 1; NRG1; Pro NRG1; SMDF

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

NRG1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human NRG1, different from the related rat and mouse sequences by one amino acids.

计算分子量

70,65,40,25 kDa

实际分子量

100,65,40 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

NRG1(Neuregulin 1), also known as ARIA, NDF or HRGA, is a protein that in humans is encoded by the NRG1 gene. NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. By in situ hybridization of a tritium-labeled probe to human metaphase spreads, Orr-Urtreger et al.(1993) localized the NDF gene to 8p21-p12. In mouse embryos 14.5 days postcoitum, Orr-Urtreger et al.(1993) found that NDF expression is confined predominantly to the central and peripheral nervous systems, including the neuroepithelium that lines the lateral ventricles of the brain, the ventral horn of the spinal cord, and the intestinal as well as dorsal root ganglia.

Uniprot ID

Q02297

文献引用格式

NRG1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3235)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

neuregulin 1

蛋白名全称

Pro-neuregulin-1, membrane-bound isoform

实验方案>

实验步骤

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