Anti-PSD95/DLG4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF

BA3304

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (BA3304). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: human U-87 MG whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PSD95/DLG4 antigen affinity purified polyclonal antibody (BA3304) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PSD95/DLG4 at approximately 95-100 kDa. The expected band size for PSD95/DLG4 is at 80 kDa.
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IHC analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (BA3304).
PSD95/DLG4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PSD95/DLG4 Antibody (BA3304) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (BA3304).
PSD95/DLG4 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PSD95/DLG4 Antibody (BA3304) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IF analysis using anti- DLG4 antibody (BA3304). detected in paraffin-embedded section of rat brain tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).
all(4)

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产品名称

Anti-PSD95/DLG4 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Disks large homolog 4; DLG4; PSD 95; PSD95; PSD95-Specific; DLG4; SAP 90; SAP90; Synapse associated protein 90

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF

基因名称

DLG4

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human PSD95, identical to the related rat and mouse sequences.

计算分子量

80 kDa

实际分子量

95-100 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

DLG4 discs large homolog 4, also known as PSD95 or SAP-90, is a protein that in humans is encoded by the DLG4 gene. It is a member of the membrane-associated guanylate kinase(MAGUK) family. This gene is mapped to 17p13.1. DLG4 can heteromultimerize with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Overexpression of DLG4 in hippocampal neurons could drive maturation of glutamatergic synapses. DLG4 can orchestrate synaptic development and it has a role in synapse stabilization and plasticity. Ubiquitination of DLG4 through an MDM2-mediated pathway can regulate AMPA receptor surface expression during synaptic plasticity.

Uniprot ID

P78352

文献引用格式

PSD95/DLG4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3304)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

discs, large homolog 4(Drosophila)

蛋白名全称

Disks large homolog 4

实验方案>

实验步骤

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