Western blot (WB): | 1:2000 |
Western blot analysis of anti-PSD95/DLG4 antibody (M02128-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-PSD95/DLG4 antigen affinity purified monoclonal antibody (M02128-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PSD95/DLG4 at approximately 95 kDa. The expected band size for PSD95/DLG4 is at 80 kDa.
Western blot analysis of anti-PSD95/DLG4 antibody (M02128-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-PSD95/DLG4 antigen affinity purified monoclonal antibody (M02128-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PSD95/DLG4 at approximately 95 kDa. The expected band size for PSD95/DLG4 is at 80 kDa.