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Anti-Alpha Antichymotrypsin/SERPINA3 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA3355

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of Alpha Antichymotrypsin/SERPINA3 using anti-Alpha Antichymotrypsin/SERPINA3 antibody (BA3355). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human SMMC whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alpha Antichymotrypsin/SERPINA3 antigen affinity purified polyclonal antibody (BA3355) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alpha Antichymotrypsin/SERPINA3 at approximately 50-60 kDa. The expected band size for Alpha Antichymotrypsin/SERPINA3 is at 48 kDa.

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  • IHC analysis of Alpha Antichymotrypsin/SERPINA3 using anti-Alpha Antichymotrypsin/SERPINA3 antibody (BA3355).
    Alpha Antichymotrypsin/SERPINA3 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Alpha Antichymotrypsin/SERPINA3 Antibody (BA3355) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(2)

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产品名称
Anti-Alpha Antichymotrypsin/SERPINA3 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
A1 Antichymotrypsin; A-1-Antichymotrypsin; AACT; ACT; Alpha 1 antichymotrypsin; GIG24; GIG25; Serpin A3; SERPINA3
产品类型
Polyclonal
检验物种
human
应用范围
WB, IHC
基因名称
SERPINA3
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence in the middle region of human alpha 1 Antichymotrypsin.
计算分子量
48 kDa
实际分子量
50-60 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Alpha 1-antichymotrypsin, also called serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 3 or GIG24 is an alpha globulin glycoprotein that is a member of the serpin superfamily. In humans, it is encoded by the SERPINA3 gene. This gene is mapped to 14q32.13. The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.
Uniprot ID
P01011  
文献引用格式
Alpha Antichymotrypsin/SERPINA3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3355)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 3
蛋白名全称
Alpha-1-antichymotrypsin
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Alpha Antichymotrypsin/SERPINA3 Antibody

    筛选器: All WB IHC

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