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Anti-Integrin Alpha 6/ITGA6 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA3356

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of Integrin Alpha 6/ITGA6 using anti-Integrin Alpha 6/ITGA6 antibody (BA3356). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human 293T whole cell lysates,
    Lane 2: human A431 whole cell lysates,
    Lane 3: human Hela whole cell lysates,
    Lane 4: human Jurkat whole cell lysates,
    Lane 5: human Raji whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Integrin Alpha 6/ITGA6 antigen affinity purified polyclonal antibody (BA3356) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Integrin Alpha 6/ITGA6 at approximately 127 kDa. The expected band size for Integrin Alpha 6/ITGA6 is at 127 kDa.

    all(2)
  • ICC analysis of Integrin Alpha 6/ITGA6 using anti-Integrin Alpha 6/ITGA6 antibody (BA3356).
    Integrin Alpha 6/ITGA6 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Integrin Alpha 6/ITGA6 Antibody (BA3356) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(2)

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产品名称
Anti-Integrin Alpha 6/ITGA6 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CD49f; Integrin alpha 6; Integrin alpha-6; integrin; alpha 6; ITGA6; ITGA6B; VLA 6
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
ITGA6
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human Integrin alpha 6, different from the related mouse and rat sequences by one amino acid.
计算分子量
127 kDa
实际分子量
127 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Integrin alpha-6, also called VLA-6 or ITGA6B is a protein that in humans is encoded by the ITGA6 gene. This gene is mapped to 2q31.1. The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome.
Uniprot ID
P23229  
文献引用格式
Integrin Alpha 6/ITGA6 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3356)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
integrin, alpha 6
蛋白名全称
Integrin alpha-6
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Integrin Alpha 6/ITGA6 Antibody

    筛选器: All WB IHC

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