Anti-HSD17B1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

BA3399

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of HSD17B1 using anti-HSD17B1 antibody (BA3399). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human T47D whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSD17B1 antigen affinity purified polyclonal antibody (BA3399) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSD17B1 at approximately 37 kDa. The expected band size for HSD17B1 is at 35 kDa.
all(5)
IHC analysis of HSD17B1 using anti-HSD17B1 antibody (BA3399).
HSD17B1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-HSD17B1 Antibody (BA3399) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of HSD17B1 using anti-HSD17B1 antibody (BA3399).
HSD17B1 was detected in a paraffin-embedded section of rat ovary tissue. The tissue section was incubated with rabbit anti-HSD17B1 Antibody (BA3399) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of HSD17B1 using anti-HSD17B1 antibody (BA3399).
HSD17B1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-HSD17B1 Antibody (BA3399) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
ICC analysis of HSD17B1 using anti- HSD17B1 antibody (BA3399).
HSD17B1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-HSD17B1 Antibody (BA3399) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)

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产品简介>

产品名称

Anti-HSD17B1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

17 beta HSD 1; 20 alpha HSD; E17KSR; E2DH; EDH17B1; EDH17B2; EDHB17; HSD17; HSD17B1; SDR28C1

产品类型

Polyclonal

检验物种

human, rat

应用范围

WB, IHC, ICC/IF

基因名称

HSD17B1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1, different from the related rat and mouse sequences by one amino acid.

计算分子量

35 kDa

实际分子量

37 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Estradiol 17-beta-dehydrogenase 1 is an enzyme that in humans is encoded by the HSD17B1 gene. This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants.

Uniprot ID

P14061

文献引用格式

HSD17B1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3399)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

hydroxysteroid(17-beta) dehydrogenase 1

蛋白名全称

Estradiol 17-beta-dehydrogenase 1

实验方案>

实验步骤

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