Anti-NR4A1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA3693-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of NR4A1 using anti-NR4A1 antibody (BA3693-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: HELA whole cell lysates,
Lane 3: JURKAT whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NR4A1 antigen affinity purified polyclonal antibody (BA3693-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NR4A1 at approximately 67 kDa. The expected band size for NR4A1 is at 64 kDa.
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产品简介>

产品名称

Anti-NR4A1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Early response protein NAK1; GFRP1; HMR; N10; NAK 1; NAK1; NGFIB; NR4A1; NUR77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST 59; Testicular receptor 3; TR3

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

NR4A1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human NUR77, identical to the related rat and mouse sequences.

计算分子量

64 kDa

实际分子量

67 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

NR4A1(NUCLEAR RECEPTOR SUBFAMILY 4, GROUP A, MEMBER 1), also called NAK1, GFRP1, TR3, NUR77 or NGFIB, is a protein that in humans is encoded by the NR4A1 gene, which is also a member of the Nur nuclear receptor family of intracellular transcription factors. The NR4A1 gene is mapped on 12q13.13. NR4A1 is involved in cell cycle mediation, inflammation and apoptosis. It plays a key role in mediating inflammatory responses in macrophages. In addition, subcellular localization of the NR4A1 protein appears to play a key role in the survival and death of cells. Nr4a1 was overexpressed in Wnt1 -transformed mouse mammary cells. Nr4a1 was also induced by lithium, a Wnt1 mimic, and the Nr4a1 promoter was activated by lithium and beta-catenin, a Wnt1 downstream effector. In contrast, human NR4A1 was not upregulated by beta-catenin, indicating that this gene is regulated differently in human and mouse cells. Adenoviral expression of Nr4a1 induced genes involved in gluconeogenesis, stimulated glucose production both in vitro and in vivo, and raised blood glucose levels.

Uniprot ID

P22736

文献引用格式

NR4A1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3693-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

nuclear receptor subfamily 4, group A, member 1

蛋白名全称

Nuclear receptor subfamily 4 group A member 1

实验方案>

实验步骤

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