Anti-RIPK2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA3824-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of RIPK2 using anti-RIPK2 antibody (BA3824-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: HELA whole cell lysates,
Lane 3: PANC whole cell lysates,
Lane 4: COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RIPK2 antigen affinity purified polyclonal antibody (BA3824-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RIPK2 at approximately 61 kDa. The expected band size for RIPK2 is at 61 kDa.
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产品名称

Anti-RIPK2 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CARD3; CARDIAK; CCK; GIG30; Receptor interacting protein 2; RICK; RIP 2; RIP2; RIPK2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

RIPK2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human RIP2, different from the related rat and mouse sequences by one amino acid.

计算分子量

61 kDa

实际分子量

61 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

RIPK2(Receptor-interacting serine/threonine-protein kinase 2), also known as CARD3, CARDIAK, RICK, RIP2, is an enzyme that in humans is encoded by the RIPK2 gene. It has 540-amino acid protein in length. Northern blot analysis revealed that RICK is expressed in various human tissues as 2.5- and 1.8-kb mRNAs that differ due to alternative polyadenylation. RICK is a novel kinase that may regulate apoptosis induced by the FAS receptor pathway. This gene encodes a member of the receptor-interacting protein(RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal°Caspase recruitment domain(CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappa B and inducer of apoptosis in response to various stimuli, CARDIAK(CARD-containing ICE-associated kinase) specifically interacted with the CARD of ICE/caspase-1, and this interaction correlated with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 subunit.

Uniprot ID

O43353

文献引用格式

RIPK2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3824-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

receptor-interacting serine-threonine kinase 2

蛋白名全称

Receptor-interacting serine/threonine-protein kinase 2

实验方案>

实验步骤

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