Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: human SKOV3 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human 22RV1 whole cell lysates.
\After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (BA3863) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates,
Lane 2: rat NRK whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: rat RH-35 whole cell lysates,
Lane 5: mouse ANA-1 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates,
Lane 8: mouse Hepa1/6 whole cell lysates.
\After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (BA3863) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human glioma tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of HL-60 cells using anti-B23/NPM1 antibody (BA3863).
Overlay histogram showing HL-60 cells stained with BA3863 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: human SKOV3 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human 22RV1 whole cell lysates.
\After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (BA3863) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates,
Lane 2: rat NRK whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: rat RH-35 whole cell lysates,
Lane 5: mouse ANA-1 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates,
Lane 8: mouse Hepa1/6 whole cell lysates.
\After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (BA3863) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human glioma tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of HL-60 cells using anti-B23/NPM1 antibody (BA3863).
Overlay histogram showing HL-60 cells stained with BA3863 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: human SKOV3 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human 22RV1 whole cell lysates.
\After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (BA3863) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates,
Lane 2: rat NRK whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: rat RH-35 whole cell lysates,
Lane 5: mouse ANA-1 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates,
Lane 8: mouse Hepa1/6 whole cell lysates.
\After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (BA3863) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human glioma tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of HL-60 cells using anti-B23/NPM1 antibody (BA3863).
Overlay histogram showing HL-60 cells stained with BA3863 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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