Anti-BAFF/TNFSF13B Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA4037

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of BAFF/TNFSF13B using anti-BAFF/TNFSF13B antibody (BA4037). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HL60 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BAFF/TNFSF13B antigen affinity purified polyclonal antibody (BA4037) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BAFF/TNFSF13B at approximately 31 kDa. The expected band size for BAFF/TNFSF13B is at 31 kDa.
all(2)
IHC analysis of BAFF/TNFSF13B using anti-BAFF/TNFSF13B antibody (BA4037).
BAFF/TNFSF13B was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BAFF/TNFSF13B Antibody (BA4037) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品名称

Anti-BAFF/TNFSF13B Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

TNF superfamily member 13b; BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; TNLG7A; ZTNF4; TNFSF13B

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

TNFSF13B

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human BAFF, different from the related rat and mouse sequences by three amino acids.

计算分子量

31 kDa

实际分子量

31 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system(CNS) lymphomas. The TNF superfamily member B cell-activating factor(BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology. The B cell activating factor BAFF(BlyS/TALL-1/zTNF4) is a tumor necrosis factor(TNF)-related ligand that promotes B cell survival and binds to three receptors(BCMA, TACI, and the recently described BAFF-R). Human BAFF was mapped to chromosome 13q32-34.4. The standard used in this kit is recombinant soluble human BAFF(A134-L295) with the molecular mass of 19.6KDa.

Uniprot ID

Q9Y275

文献引用格式

BAFF/TNFSF13B Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA4037)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

tumor necrosis factor(ligand) superfamily, member 13b

蛋白名全称

Tumor necrosis factor ligand superfamily member 13B

实验方案>

实验步骤

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