Western blot (WB): | 1:500-2000 |
Western blot analysis of BAFF/TNFSF13B using anti-BAFF/TNFSF13B antibody (PB0553). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Kidney tissue lysates,
Lane 2: Rat Spleen tissue lysates,
Lane 3: Mouse Spleen tissue lysates,
Lane 4: Human Placenta tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BAFF/TNFSF13B antigen affinity purified polyclonal antibody (PB0553) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BAFF/TNFSF13B at approximately 31 kDa. The expected band size for BAFF/TNFSF13B is at 31 kDa.
Western blot analysis of BAFF/TNFSF13B using anti-BAFF/TNFSF13B antibody (PB0553). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Kidney tissue lysates,
Lane 2: Rat Spleen tissue lysates,
Lane 3: Mouse Spleen tissue lysates,
Lane 4: Human Placenta tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BAFF/TNFSF13B antigen affinity purified polyclonal antibody (PB0553) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BAFF/TNFSF13B at approximately 31 kDa. The expected band size for BAFF/TNFSF13B is at 31 kDa.