Anti-Trefoil Factor 1/TFF1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA4923

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Trefoil Factor 1/TFF1 using anti-Trefoil Factor 1/TFF1 antibody (BA4923). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat gaster tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Trefoil Factor 1/TFF1 antigen affinity purified polyclonal antibody (BA4923) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Trefoil Factor 1/TFF1 at approximately 12 kDa. The expected band size for Trefoil Factor 1/TFF1 is at 10 kDa.
all(2)
IHC analysis of Trefoil Factor 1/TFF1 using anti-Trefoil Factor 1/TFF1 antibody (BA4923).
Trefoil Factor 1/TFF1 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-Trefoil Factor 1/TFF1 Antibody (BA4923) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品名称

Anti-Trefoil Factor 1/TFF1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

D21S21; HP1.A; HPS2; pNR 2; Polypeptide P1.A; Protein pS2; pS2; TFF1; trefoil factor 1

产品类型

Polyclonal

检验物种

rat

应用范围

WB, IHC

基因名称

TFF1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of mouse Estrogen Inducible Protein pS2, identical to the related rat sequence.

计算分子量

10 kDa

实际分子量

12 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

TFF1(Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

Uniprot ID

Q08423

文献引用格式

Trefoil Factor 1/TFF1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA4923)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

trefoil factor 1

蛋白名全称

Trefoil factor 1

实验方案>

实验步骤

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