Western blot analysis of anti- Pan cytokeratin antibody (BM0030). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCL-H460 whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: A431 whole cell lysates,
Lane 4: mouse skin tissue lysates.
Use mouse anti- Pan cytokeratin 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Pan cytokeratin at approximately 54KD. The expected band size for Pan cytokeratin is at 54KD.
IHC analysis using anti- Pan cytokeratin antibody (BM0030). detected in paraffin-embedded section of human Lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(3) 
IF analysis using anti- Pan cytokeratin antibody (BM0030). detected in paraffin-embedded section of human intestine cancer tissue. The tissue section were stained using the FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green)(Catalog#BA1101) .
all(3) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of anti- Pan cytokeratin antibody (BM0030). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCL-H460 whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: A431 whole cell lysates,
Lane 4: mouse skin tissue lysates.
Use mouse anti- Pan cytokeratin 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Pan cytokeratin at approximately 54KD. The expected band size for Pan cytokeratin is at 54KD.
IHC analysis using anti- Pan cytokeratin antibody (BM0030). detected in paraffin-embedded section of human Lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
IF analysis using anti- Pan cytokeratin antibody (BM0030). detected in paraffin-embedded section of human intestine cancer tissue. The tissue section were stained using the FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green)(Catalog#BA1101) .
Western blot analysis of anti- Pan cytokeratin antibody (BM0030). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCL-H460 whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: A431 whole cell lysates,
Lane 4: mouse skin tissue lysates.
Use mouse anti- Pan cytokeratin 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Pan cytokeratin at approximately 54KD. The expected band size for Pan cytokeratin is at 54KD.
IHC analysis using anti- Pan cytokeratin antibody (BM0030). detected in paraffin-embedded section of human Lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
IF analysis using anti- Pan cytokeratin antibody (BM0030). detected in paraffin-embedded section of human intestine cancer tissue. The tissue section were stained using the FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green)(Catalog#BA1101) .
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